Liquid Chromatography on the Different Methods for the Determination of Lipophilicity: An Essential Analytical Tool in Medicinal Chemistry

Lipophilicity is one of many parameters involved in the biological activity drugs, as it affects their pharmacokinetic and pharmacodynamic behavior. Generally, lipophilicity assessed by partition coefficient a compound between nonpolar phase (n-octanol) an aqueous (water), expressed P (partition coefficient) or its decimal logarithm (Log P). The gold standard method for experimental determination Log shake-flask method. In this context, chromatographic methods enable direct simple quantification partitioned two phases. This review discusses use liquid chromatography (LC) indirect lipophilicity. Beyond classical isotropic log determination, assessing anisotropic are also reviewed. Several examples discussed that highlight versatility LC technique current trends. last section focuses on case study describing experience our group emphasizing dual role determining P.